BioMycoX® Mycoplasma PCR Detection Kit
BioMycoX® Mycoplasma Detection kit utilizes the polymerase chain reaction (PCR), which is the method of choice for highest sensitivity in the detection of Mycoplasma contamination in cell cultures and other cell culture derived biologicals. The primer set is specific to the highly conserved 16S rRNA coding region in the mycoplasma genome. This allows the detection of M. orale, M. hyorhinis, M. arginini, M. fermentans, Acholeplasma laidlawii, M. hominis usually encountered as contaminants in cell cultures. Furthermore, this kit can detect M. pneumoniae, M. salivarium, M. synoviae and Ureaplasma species. Eukaryotic and bacterial DNA is not amplified by BioMycoX® Mycoplasma Detection kit.
| Cat. No | D-25, D-50 and D-100 (D-Number of Sample ) |
| Storage & Stability | -20°C |
| Intended Use | Screening of mycoplasma contamination in R&D cell cultures |
| Method | Conventional PCR |
| Sample Material | Template DNA prepared by boiling of culture supernatant |
| Sensitivity | 10~100 fg/reaction |
Characteristics of CellSafe’s Mycoplasma Detection Kit
CellSafe’s Mycoplasma Detection kits utilize the PCR technique which is established as the method of choice for the highest sensitivity in the detection of mycoplasma contamination in cell culture and other cell culture derived biologicals. Detection of M.orale, M.hyorhinis, M.arginini, M.fermentans, M.hominis and Acholeplasma laidlawii usually encountered as contaminants in cell cultures. Furthermore, these kits can detect M.pneumoniae, M.salivarium, M.genitalium, M.penentrans and Ureaplasma species. The primers are specific to the highly conserved 16s rRNA coding region and the kits do not detect other bacteria and cell-lines. All kits include Internal Amplification Control (IAC) providing validity of test results. They are user firendly, ready-to-use, optimized PCR master mix type kits.
BioMycoX® Mycoplasma PCR Detection Kit
• Screening of mycoplasma contamination in R&D cell cultures
• Conventional PCR Type
• Template DNA is prepared by boiling of culture supernatant
• Sensitivity: 10~100 fg/reaction
• The results are within 2~3 hours
