BioMycoX® Mycoplasma qPCR Detection Kit
The BioMycoX® Mycoplasma qPCR Detection Kit is used to detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) using Probe. The BioMycoX® Mycoplasma qPCR Detection Kit includes a Primer and FAM labeled probe specific for mycoplasma species and Hex labeled probe specific for internal control DNA. The primer set is specific to the highly conserved 16S rRNA coding region in the mycoplasma genome. This allows the detection of M. orale, M. hyorhinis, M. arginini, M. fermentans, Acholeplasma laidlawii, M. hominis, usually encountered as contaminants in cell cultures. Furthermore, this kit can detect M. pneumoniae, M. salivarium, M. synoviae and Ureaplasma species. Eukaryotic and bacterial DNA is not amplified by BioMycoX® Mycoplasma qPCR Detection Kit.
| Cat. No | QDR-25, QDR-50, QDR-100 (QDR-Number of Sample) |
| Storage | -20°C |
| Intended Use | Screening of mycoplasma contamination in R&D cell cultures |
| Method | Real-Time PCR |
| Sample Material | Template DNA prepared by boiling of culture supernatant |
| Sensitivity | 10~100 fg/reaction |
Characteristics of CellSafe’s Mycoplasma Detection Kit
CellSafe’s Mycoplasma Detection kits utilize the PCR technique which is established as the method of choice for the highest sensitivity in the detection of mycoplasma contamination in cell culture and other cell culture derived biologicals. Detection of M.orale, M.hyorhinis, M.arginini, M.fermentans, M.hominis and Acholeplasma laidlawii usually encountered as contaminants in cell cultures. Furthermore, these kits can detect M.pneumoniae, M.salivarium, M.genitalium, M.penentrans and Ureaplasma species. The primers are specific to the highly conserved 16s rRNA coding region and the kits do not detect other bacteria and cell-lines. All kits include Internal Amplification Control (IAC) providing validity of test results. They are user firendly, ready-to-use, optimized PCR master mix type kits.
BioMycoX® Mycoplasma qPCR Detection Kit
The BioMycoX® Mycoplasma qPCR Detection Kit is used to detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) using Probe. The BioMycoX® Mycoplasma qPCR Detection Kit includes a Primer and Probe mixes. These mixes contain FAM labeled probe specific for mycoplasma species and Hex labeled probe for internal control DNA. The primer set is specific to the highly conserved the 16S rRNA coding region in the mycoplasma genome. This allows the detection of M. orale, M. hyorhinis, M. arginini, M. fermentans, Acholeplasma laidlawii, M. hominis, usually encountered as contaminants in cell cultures. Furthermore, this kit can detect M. pneumoniae, M. salivarium, M. synoviae and Ureaplasma species. Eukaryotic and bacterial DNA is not amplified by BioMycoX® Mycoplasma qPCR Detection Kit.
The BioMycoX® Mycoplasma qPCR Detection Kit is capable of detecting Mycoplasma infections in cell cultures in less than three hours, depending on the spectrofluorometric thermal cycler used for detection.
Kit Specificity
The BioMycoX® Mycoplasma qPCR Detection Kit detect Mycoplasma species simply, reliably, and rapidly. To detect the presence of these microorganisms, the assay uses the polymerase chain reaction (PCR) to amplify a target unique to a wide variety of mycoplasmas. The kit can detect almost all kinds of Mycoplasma species, including Acholeplasma laidlawii and Spiroplasma citri. The kit does not detect other genera or cell-line DNA.
Kit Sensitivity
The sensitivity of the PCR using this kit is 1 to 100 copies of the target DNA per reaction. Sensitivity of the assay in real culture samples depends on the quality of the sample preparation.
